Journal: PLoS ONE

Article Title: Chondroitin Sulfate Proteoglycan CSPG4 as a Novel Hypoxia-Sensitive Marker in Pancreatic Tumors

doi: 10.1371/journal.pone.0100178

Figure Lengend Snippet: Immunoblotting confirmed the specificity of polyclonal H-300 ( A ) and monoclonal LHM2 antibodies ( B ), generated against recombinant protein and melanoma-derived antigens, respectively. The images demonstrate differences in size between pancreatic (band-2) and melanoma (SK-MEL-28, band-1) antigens, but not cervical carcinoma (HeLa) antigens ( C ), and reveal the frequent existence of oversized isoforms in SCA and PDAC (band 3, C–D ). GAPDH was used as the loading control. HiMark (Invitrogen) was used as the molecular weight marker. ( E ) Western blot and ( F ) FACS analyses of CSPG4 siRNA-transfected Panc1 cells further confirmed the specificity of the used antibodies and established the knockdown efficacy of the two siRNA sets (si1 and si2) at approximately 75% after 48 h post transfection, compared to the negative control siRNA (neg.si). QRT-PCR confirmed the efficacy of the knockdowns at the mRNA level.

Article Snippet: The USCN/Cloud-Clone kit employed a recombinant N-terminal fragment of a protein (Leu16-Ser350) as an ELISA standard and immunogen to produce mouse monoclonal (capture) and goat polyclonal (detection) antibodies; CUSABIO - a full-length protein expressed in eukaryotic cells.

Techniques: Western Blot, Generated, Recombinant, Derivative Assay, Control, Molecular Weight, Marker, Transfection, Knockdown, Negative Control, Quantitative RT-PCR

Journal: Developmental cell

Article Title: In vitro and in vivo development of the human airway at single cell resolution

doi: 10.1016/j.devcel.2020.01.033

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit anti-EGFR , Sigma-Aldrich Atlas Antibodies , HPA018530.

Techniques: Transduction, Control, Virus, Plasmid Preparation, Recombinant, RNAscope, Multiplex Assay, In Situ Hybridization, Generated, Software

Journal: bioRxiv

Article Title: Unveiling DNA Origami Interaction Dynamics on Living Cell Surfaces by Single Particle Tracking

doi: 10.1101/2024.12.23.628980

Figure Lengend Snippet: a) Schematic on the DNA origami self-assembly approach, combining the p7560 viral scaffold, the staple strands (core staples, 18 staples with extended handles for antibody/aptamer functionalization and 6 staples with extended handles for fluorescent dye functionalization). Folding and purification of the NR via poly-ethylene glycol precipitation was confirmed by agarose gel electrophoresis. b) Schematic representation of anti-EGFR conjugation approach to a ssDNA oligonucleotide, via click chemistry, and to Cy3, via NHS-ester. A native PAGE shows further migration of the oligo conjugated antibody towards the positive pole (lane 2). c) Conjugation of the anti-EGFR antibodies or aptamers to the NRs. The agarose gel shows differences the migration height of the different NRs, confirming their correct functionalization. Legend: Sc=Scaffold, NR=Folded DNA origami nanorod, NR_18Ab= NRs functionalized with anti-EGFR antibody, and NR_18Apt= NR functionalized with anti-EGFR aptamers. d) Atomic force microscopy images of the non-functionalized NRs (upper panel) and the NRs coated with 18 antibodies (lower panel).

Article Snippet: P7560 DNA scaffold (Tilibit nanosystems, Germany), Staples and modified staples (Integrated DNA technologies Europe), PEG8000 (Sigma-Aldrich, USA), InVivoMab anti-human EGFR antibody (Bio X cell, USA), NHS ester-(PEG) 4 -DBCO (Vector Labs, USA), Cyanine3 NHS ester (Lumiprobe, USA), Amicon 10 and 100 kDa weight cut-off (Sigma-Aldrich, USA), mini-PROTEAN TGX precast gels (BIO-RAD Laboratories Inc, USA)

Techniques: Purification, Agarose Gel Electrophoresis, Conjugation Assay, Clear Native PAGE, Migration, Microscopy

Journal: bioRxiv

Article Title: Unveiling DNA Origami Interaction Dynamics on Living Cell Surfaces by Single Particle Tracking

doi: 10.1101/2024.12.23.628980

Figure Lengend Snippet: non-denaturing SDS page gel of the EGFR antibody (first lane) and the oligo conjugated EGFR antibody (lane 2-5, increasing concentration of antibody)

Article Snippet: P7560 DNA scaffold (Tilibit nanosystems, Germany), Staples and modified staples (Integrated DNA technologies Europe), PEG8000 (Sigma-Aldrich, USA), InVivoMab anti-human EGFR antibody (Bio X cell, USA), NHS ester-(PEG) 4 -DBCO (Vector Labs, USA), Cyanine3 NHS ester (Lumiprobe, USA), Amicon 10 and 100 kDa weight cut-off (Sigma-Aldrich, USA), mini-PROTEAN TGX precast gels (BIO-RAD Laboratories Inc, USA)

Techniques: SDS Page, Concentration Assay

Journal: bioRxiv

Article Title: Unveiling DNA Origami Interaction Dynamics on Living Cell Surfaces by Single Particle Tracking

doi: 10.1101/2024.12.23.628980

Figure Lengend Snippet: Immunostaining of the EGFR on Hek 293T cells (left) and the MDA MD 468 cells (right). Images were taken via confocal laser scanning microscopy.

Article Snippet: P7560 DNA scaffold (Tilibit nanosystems, Germany), Staples and modified staples (Integrated DNA technologies Europe), PEG8000 (Sigma-Aldrich, USA), InVivoMab anti-human EGFR antibody (Bio X cell, USA), NHS ester-(PEG) 4 -DBCO (Vector Labs, USA), Cyanine3 NHS ester (Lumiprobe, USA), Amicon 10 and 100 kDa weight cut-off (Sigma-Aldrich, USA), mini-PROTEAN TGX precast gels (BIO-RAD Laboratories Inc, USA)

Techniques: Immunostaining, Confocal Laser Scanning Microscopy

Journal: bioRxiv

Article Title: Unveiling DNA Origami Interaction Dynamics on Living Cell Surfaces by Single Particle Tracking

doi: 10.1101/2024.12.23.628980

Figure Lengend Snippet: Agarose gel of nanorods conjugated with 8 or 18 EGFR antibodies and 8 or 18 aptamers.

Article Snippet: P7560 DNA scaffold (Tilibit nanosystems, Germany), Staples and modified staples (Integrated DNA technologies Europe), PEG8000 (Sigma-Aldrich, USA), InVivoMab anti-human EGFR antibody (Bio X cell, USA), NHS ester-(PEG) 4 -DBCO (Vector Labs, USA), Cyanine3 NHS ester (Lumiprobe, USA), Amicon 10 and 100 kDa weight cut-off (Sigma-Aldrich, USA), mini-PROTEAN TGX precast gels (BIO-RAD Laboratories Inc, USA)

Techniques: Agarose Gel Electrophoresis

Journal: Cancers

Article Title: Analysis of the Single-Cell Heterogeneity of Adenocarcinoma Cell Lines and the Investigation of Intratumor Heterogeneity Reveals the Expression of Transmembrane Protein 45A (TMEM45A) in Lung Adenocarcinoma Cancer Patients

doi: 10.3390/cancers14010144

Figure Lengend Snippet: Antibodies used for mass cytometry.

Article Snippet: 3170009B , Fluidigm , EGFR , 170 Er.

Techniques: Cytometry

Journal: Frontiers in Oncology

Article Title: Intrinsic Differences in Spatiotemporal Organization and Stromal Cell Interactions Between Isogenic Lung Cancer Cells of Epithelial and Mesenchymal Phenotypes Revealed by High-Dimensional Single-Cell Analysis of Heterotypic 3D Spheroid Models

doi: 10.3389/fonc.2022.818437

Figure Lengend Snippet: List of IMC antibodies.

Article Snippet: 142 Nd , EGFR , D38B1 , 3142013D , Fluidigm , 1:50.

Techniques: